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Abstract This study uses a small unmanned aircraft system equipped with a multispectral sensor to assess various vegetation indices (VIs) for their potential to monitor iron deficiency chlorosis (IDC) in a grain sorghum (Sorghum bicolorL.) crop. IDC is a nutritional disorder that stunts a plants’ growth and causes its leaves to yellow due to an iron deficit. The objective of this project is to find the best VI to detect and monitor IDC. A series of flights were completed over the course of the growing season and processed using Structure‐from‐Motion photogrammetry to create orthorectified, multispectral reflectance maps in the red, green, red‐edge, and near‐infrared wavelengths. Ground data collection methods were used to analyze stress, chlorophyll levels, and grain yield, correlating them to the multispectral imagery for ground control and precise crop examination. The reflectance maps and soil‐removed reflectance maps were used to calculate 25 VIs whose separability was then calculated using a two‐class distance measure, determining which contained the largest separation between the pixels representing IDC and healthy vegetation. The field‐acquired data were used to conclude which VIs achieved the best results for the dataset as a whole and at each level of IDC (low, moderate, and severe). It was concluded that the MERIS terrestrial chlorophyll index, normalized difference red‐edge, and normalized green (NG) indices achieved the highest amount of separation between plants with IDC and healthy vegetation, with the NG reaching the highest levels of separability for both soil‐included and soil‐removed VIs. 

Abstract We present the in-lab and on-sky performance for the upgraded 90 GHz focal plane of the Cosmology Large Angular Scale Surveyor, which had four of its seven detector wafers updated during the austral winter of 2022. The update aimed to improve the transition-edge-sensor (TES) stability and bias range and to realize the high optical efficiency of the sensor design. Modifications included revised circuit terminations, electrical contact between the TES superconductor and the normal metal providing the bulk of the bolometer heat capacity, and additional filtering on the TES bias lines. The upgrade was successful: 94% of detectors are stable down to 15% of the normal resistance, providing a wide overlapping range of bias voltages for all TESs on a wafer. The median telescope efficiency improved from $0.42_{-0.22}^{+0.15}$to $0.60_{-0.32}^{+0.10}$(68% quantiles). For the four upgraded wafers alone, median telescope efficiency increased to $0.65_{-0.06}^{+0.06}$. Given our efficiency estimate for the receiver optics, this telescope efficiency implies a detector efficiency exceeding 0.90. The overall noise-equivalent temperature of the 90 GHz focal plane improved from $19\mu \mathrm{K}\sqrt{\mathrm{s}}$to $9.7\mu \mathrm{K}\sqrt{\mathrm{s}}$. 

Although the gut and the brain vastly differ in physiological function, they have been interlinked in a variety of different neurological and behavioral disorders. The bacteria that comprise the gut microbiome communicate and influence the function of various physiological processes within the body, including nervous system function. However, the effects of social experience in the context of dominance and social stress on gut microbiome remain poorly understood. Here, we examined whether social experience impacts the host zebrafish (Danio rerio) gut microbiome. We studied how social dominance during the first 2 weeks of social interactions changed the composition of zebrafish gut microbiome by comparing gut bacterial composition, diversity, and relative abundance between socially dominant, submissive, social isolates and control group–housed communal fish. Using amplicon sequencing of the 16S rRNA gene, we report that social dominance significantly affects host gut bacterial community composition but not bacterial diversity. At the genus level, Aeromonas and unclassified Enterobacteriaceae relative abundance decreased in dominant individuals while commensal bacteria (e.g., Exiguobacterium and Cetobacterium) increased in relative abundance. Conversely, the relative abundance of Psychrobacter and Acinetobacter was increased in subordinates, isolates, and communal fish compared to dominant fish. The shift in commensal and pathogenic bacteria highlights the impact of social experience and the accompanying stress on gut microbiome, with potentially similar effects in other social organisms. 

Front-end polarization modulation enables improved polarization measurement stability by modulating the targeted signal above the low-frequency $1/f$ drifts associated with atmospheric and instrumental instabilities and diminishes the impact of instrumental polarization. In this work, we present the design and characterization of a new 60-cm diameter Reflective Half-Wave Plate (RHWP) polarization modulator for the 90 GHz band telescope of the Cosmology Large Angular Scale Surveyor (CLASS) project. The RHWP consists of an array of parallel wires (diameter 50~µm, 175~µm pitch) positioned 0.88~mm from an aluminum mirror. In lab tests, it was confirmed that the wire resonance frequency ($$f_\mathrm{res}$$) profile is consistent with the target, $139$~Hz$$<154$$~Hz in the optically active region (diameter smaller than 150~mm), preventing the wire vibration during operation and reducing the RHWP deformation under the wire tension. The mirror tilt relative to the rotating axis was controlled to be $<15''$, corresponding to an increase in beam width due to beam smearing of < $0.6''$, %a beam smearing amplitude of $<0.6''$, negligible compared to the beam's full-width half-maximum of $36'$. The median and 16/84th percentile of the wire--mirror separation residual was $$0.048^{+0.013}_{-0.014}$$~mm in the optically active region, achieving a modulation efficiency $$\epsilon=96.2_{+0.5}^{-0.4}\%$$ with an estimated bandpass of 34~GHz. The angular velocity of the RHWP was maintained to an accuracy of within 0.005\% at the nominal rotation frequency (2.5~Hz). The RHWP has been successfully integrated into the CLASS 90 GHz telescope and started taking data in June 2024, replacing the previous modulator that has been in operation since June 2018. 

Abstract We present measurements of large-scale cosmic microwave backgroundE-mode polarization from the Cosmology Large Angular Scale Surveyor 90 GHz data. Using 115 det-yr of observations collected through 2024 with a variable-delay polarization modulator, we achieved a polarization sensitivity of $82\mu \mathrm{K}\mathrm{arcmin}$, comparable to Planck at similar frequencies (100 and 143 GHz ). The analysis demonstrates effective mitigation of systematic errors and addresses challenges to large-angular-scale power recovery posed by time-domain filtering in maximum-likelihood map-making. A novel implementation of the pixel-space transfer matrix is introduced, which enables efficient filtering simulations and bias correction in the power spectrum using the quadratic cross-spectrum estimator. Overall, we achieved an unbiased time-domain filtering correction to recover the largest angular scale polarization, with the only power deficit, arising from map-making nonlinearity, being characterized as <3%. Through cross-correlation with Planck, we detected the cosmic reionization at 99.4% significance and measured the reionization optical depth $\tau =0.053_{-0.019}^{+0.018}$, marking the first ground-based attempt at such a measurement. At intermediate angular scales (ℓ > 30), our results, both independently and in cross-correlation with Planck, remain fully consistent with Planck’s measurements. 

Abstract The Cosmology Large Angular Scale Surveyor (CLASS) is a telescope array that observes the cosmic microwave background (CMB) over ∼75% of the sky from the Atacama Desert, Chile, at frequency bands centered near 40, 90, 150, and 220 GHz. CLASS measures the large angular scale CMB polarization to constrain the tensor-to-scalar ratio and the optical depth to last scattering. This paper presents the optical characterization of the 90 GHz telescope. Observations of the Moon establish the pointing while dedicated observations of Jupiter are used for beam calibration. The standard deviations of the pointing error in azimuth, elevation, and boresight angle are 1.′3, 2.′1, and 2.′0, respectively, over the first 3 yr of observations. This corresponds to a pointing uncertainty ∼7% of the beam’s full width at half-maximum (FWHM). The effective azimuthally symmetrized instrument 1D beam estimated at 90 GHz has an FWHM of 0.°620 ± 0.°003 and a solid angle of 138.7 ± 0.6(stats.) ± 1.1(sys.)μsr integrated to a radius of 4°. The corresponding beam window function drops to $b_{\mathit{\ell }}^2=0.93,0.71,0.14$atℓ= 30, 100, 300, respectively. Far-sidelobes are studied using detector-centered intensity maps of the Moon and measured to be at a level of 10⁻³or below relative to the peak. The polarization angle of Tau A estimated from preliminary survey maps is 149°.6 ± 0°.2(stats.) in equatorial coordinates. The instrumental temperature-to-polarization (T→P) leakage fraction, inferred from per-detector demodulated Jupiter scan data, has a monopole component at the level of 1.7 × 10⁻³, a dipole component with an amplitude of 4.3 × 10⁻³, with no evidence of quadrupolar leakage. 

Abstract We report on the disk-averaged absolute brightness temperatures of Venus measured at four microwave frequency bands with the Cosmology Large Angular Scale Surveyor. We measure temperatures of 432.3 ± 2.8, 355.6 ± 1.3, 317.9 ± 1.7, and 294.7 ± 1.9 K for frequency bands centered at 38.8, 93.7, 147.9, and 217.5 GHz, respectively. We do not observe any dependence of the measured brightness temperatures on solar illumination for all four frequency bands. A joint analysis of our measurements with lower-frequency Very Large Array observations suggests relatively warmer (∼7 K higher) mean atmospheric temperatures and lower abundances of microwave continuum absorbers than those inferred from prior radio occultation measurements. 

Abstract Measurement of the largest angular scale (ℓ< 30) features of the cosmic microwave background (CMB) polarization is a powerful way to constrain the optical depth to reionization and search for the signature of inflation through the detection of primordialB-modes. We present an analysis of maps covering 73.6% of the sky made from the 40 GHz channel of the Cosmology Large Angular Scale Surveyor (CLASS) from 2016 August to 2022 May. Taking advantage of the measurement stability enabled by front-end polarization modulation and excellent conditions from the Atacama Desert, we show this channel achieves higher sensitivity than the analogous frequencies from satellite measurements in the range 10 <ℓ< 100. Simulations show the CLASS linear (circular) polarization maps have a white noise level of $125\left(130\right)\mu \mathrm{K}\mathrm{arcmin}$. We measure the Galaxy-maskedEEandBBspectra of diffuse synchrotron radiation and compare to space-based measurements at similar frequencies. In combination with external data, we expand measurements of the spatial variations of the synchrotron spectral energy density (SED) to include new sky regions and measure the diffuse SED in the harmonic domain. We place a new upper limit on a background of circular polarization in the range 5 <ℓ< 125 with the first bin showingD_ℓ< 0.023 $\mu {\mathrm{K}}_{\mathrm{CMB}}^2$at 95% confidence. These results establish a new standard for recovery of the largest-scale CMB polarization from the ground and signal exciting possibilities when the higher sensitivity and higher-frequency CLASS channels are included in the analysis. 

Polarization modulation is a powerful technique to increase the stability of measurements by enabling the distinction of a polarized signal from dominant slow system drifts and unpolarized foregrounds. Furthermore, when placed as close to the sky as possible, modulation can reduce systematic errors from instrument polarization. In this work, we introduce the design and preliminary drive system laboratory performance of a new 60 cm diameter reflective half-wave plate (RHWP) polarization modulator. The wave plate consists of a wire array situated in front of a flat mirror. Using 50 μm diameter wires with 175 μm spacing, the wave plate will be suitable for operation in the millimeter wavelength range with flatness of the wires and parallelism to the mirror held to a small fraction of a wavelength. The presented design targets the 77-108 GHz range. Modulation is performed by a rotation of the wave plate with a custom rotary drive utilizing an actively controlled servo motor.